Study on a 3D-Bioprinted Tissue Model of Self-Assembled Nanopeptide Hydrogels Combined With Adipose-Derived Mesenchymal Stem Cells
نویسندگان
چکیده
Objective: This study aimed to observe the cell growth status and multidirectional differentiation ability in a 3D-bioprinted tissue model of self-assembled nanopeptides human adipose-derived mesenchymal stem cells (Ad-MSCs). Methods: Primary Ad-MSCs were isolated, cultured, identified by flow cytometry. Tissue models printed via 3D bioprinting technology using “biological ink” consisting mixed solution Ad-MSCs. induced into osteogenic, adipogenic, endothelial compared with control groups staining. Results: The nanopeptide fiber was 10–30 nm diameter 200–500 length under atomic-force microscope. It had characteristics nano-scale materials. Flow cytometry showed that isolated cultured positive for CD29 (98.51%), CD90 (97.87%), CD166 (98.32%) but did not express CD31 (1.58%), CD34 (2.42%), CD45 (2.95%), or leukocyte antigen (HLA)-DR (0.53%), consistent immunophenotype Then, biological ink, followed induction within model. Alizarin red S staining formation calcium nodules osteogenesis experimental group, oil O stained lipid droplets adipogenesis whereas two stained. Conclusion: from primary cultures have cells. Self-assembled hydrogel is good engineering material can serve as an extracellular matrix. 3D-printed ink grew well still strong ability.
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ژورنال
عنوان ژورنال: Frontiers in Bioengineering and Biotechnology
سال: 2021
ISSN: ['2296-4185']
DOI: https://doi.org/10.3389/fbioe.2021.663120